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Related post: October 1, 1984 to September 30, 1985 TITLE OF PROJECT (80 characters or less. Title must tit or> one line between the borders.) Structure of Parainfluenza Type 3 Virus Genome PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laboratory. PI: Peter L. Collins, Ph.D. Others: Melanie K. Spriggs, Ph.D. Narayanasomy Elango, Ph.D. S. Venkatesan, M.D. Robert C . Jambou Kathleen Coelingh, Ph.D. Robert A. Olmsted, Ph.D. Alicia Buckler-White, Ph.D. Senior Staff Fellow Staff Fellow Visiting Associate Expert Biologist Senior Staff Fellow Staff Fellow Staff Fellow and institute affiliation) LID, NIAID LID, NIAID LID, NIAID LID, NIAID LID, Ceclor Cd 375 Mg NIAID LID, NIAID LID, NIAID LID, NIAID COOPERATING UNITS (if any) LIG, NIAID (Dr. John E. Coligan) LAB/BRANCH Laboratory of Infectious Diseases SECTION Respiratory Viruses Section INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Maryland 20205 TOTAL MAN-YEARS: 2.2 PROFESSIONAL: 1.7 OTHER: 0.5 CHECK APPROPRIATE BOX(ES) n (a) Human subjects n (a1) Minors n (a2) Interviews n (b) Human tissues C2 (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) We have constructed and identified cDNA classes of human parainfluenza virus type 3 (PI3 virus) mRNAs encoding the viral hemagglutinin-neuraminidase (HN) glycoprotein, nucleocapsid phosphoprotein (P) Buy Ceclor Cd , major nucleocapsid (N) protein, matrix (M) protein, and fusion (F) glycoprotein. Northern blot hybridization identified the corresponding major viral mRNAs extracted from PI3 virus-infected cells. cDNAs of the N, P and M mRNAs were identified by hybrid-selected translation and hybrid-arrest of translation in vitro . A cDNA clone containing the complete HN mRNA was sequenced, and the identification of this cDNA was confirmed by comparison of the predicted protein sequence with a partial sequence obtained by direct amino acid sequencing of authenic HN protein purified from infected cells. Based on partial nucleotide sequence information for the N, P, M and F cDNAs , oligodeoxynucleotides were synthesized and used to direct dideoxynucleotide sequencing of purified viral genomic RNA. To date, this analysis determined a partial order of genes on the PI3 genome: 3' N-P-M-(F)-HN-5' . Further sequence analysis of cDNAs and viral genomic RNA will provide a complete enumeration of viral genes and a complete determination of viral Ceclor Cd 375 gene sequences. 7-50 PHS 6040 (Rev. 1/84) GPO 9I4-9IS DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00324-04 LID PERIOD COVERED October 1, 1984 to September 30, 1985 TITLE OF PROJECT (80 characters or less. Title must fit on one line between the borders.) Laboratory Studies of Myxoviruses PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation) PI: Ceclor Cd Tablet Mark H. Snyder, M.D. Medical Staff Fellow LID, NIAID Others: Alicia Buckler-White, Ph.D. Brian R. Murphy, M.D. Robert M. Chanock, M.D. Staff Fellow Head, RV Section Chief LID, NIAID LID, NIAID LID, NIAID COOPERATING UNITS r/^ any) y. of Michigan (Dr. Maassab, Dr. DeBorde) LAB/BRANCH Laboratory of Infectious Diseases SECTION Respiratory Virus Section INSTITUTE AND LOCATION
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